alu pcr
Objective: To understand and determine the frequency of the Alu insert into the gel using PCR in the STEM classroom.
Materials/Reagents:
1. 0.9% Saline Solution
2. Student Cheek Cells
3. 5% Chelex
4. Primer Mix Data:
5. Mater Mix (Nucleotides, DNA Polymerase, Buffer) (++) is 715bp
6. TBE Buffer (+ -) is 415bp,715bp
7. Agarose Gel, Tracking Dye (- -) is 415bp
8. DNA Stain "Gel Red" My results: (- -)
9. Positive Control DNA
10. Base Pair Ladder
for more information look to www.babec.org
Procedure:
1. Remove tape from gel. Place in gel tank.
2. Gently remove combs.
3. Prepare samples with micropipet (P-2-20):
- 20 ml DNA and 4 microliters 6x loading dye. Spin 2 seconds in centrifuge.
4. Load samples onto gel with micropipet (P-20-200).
5. Put cover on gel tank and plug into power supply.
6. Run at 110V for approximately 45 minutes.
7. Stain for several hours with Ethidium Bromide
8. Rinse and observe with light.
results:
Analysis:
Here are my results. I am homozgous negative (-/-). This means that both of my parents have the same alu gene of (- -).
Conclusion
Isolating DNA from a solution is very valuable. It helps separate DNA and sort of purify it. Separating the DNA on a gel allows us to get a closer look and analyze the DNA more clearly. Separating DNA on gels is very beneficial to the forensics lab. Many times DNA needs to be analyzed in crimes, and it can be used as evidence. By using a gel Forensics scientists are able to compare different DNA samples according to how they show up on the gel.
Here are my results. I am homozgous negative (-/-). This means that both of my parents have the same alu gene of (- -).
Conclusion
Isolating DNA from a solution is very valuable. It helps separate DNA and sort of purify it. Separating the DNA on a gel allows us to get a closer look and analyze the DNA more clearly. Separating DNA on gels is very beneficial to the forensics lab. Many times DNA needs to be analyzed in crimes, and it can be used as evidence. By using a gel Forensics scientists are able to compare different DNA samples according to how they show up on the gel.